Our client, one of the largest Pharmaceutical companies in USA is looking for a Scientist-II (Associate) to work at South San Francisco, CA. **Job Title: Scientist II** **Duration:** 12 Month Contract **Location:** Onsite - South San Francisco, CA 94080 **Pay range: $50 - $53** **No PHD** **Key skills (should be mentioned in responsibilities of the resumes):** + **Maintain and propagate CHO cells in suspension culture independently** + **Perform transient and stable transfections for protein expression in mammalian cells(CHO cells).** + **Purification batch purification using bench top.** + **Candidate should have foundational experience in purification and QC** **Qualifications:** **Education and experience required:** + B.S. degree in Biochemistry, Cell/Molecular Biology, Bioprocess engineering or related fields and three (3) years of industry or academia experience or a M.S. degree with 1 year of experience in similar fields **Must haves:** + **Prior experience in mammalian cell culture techniques, and cell culture media preparation is a prerequisite: single use mammalian cell culture tools employed in bioprocess development, including but not limited to deep well plates and shake flasks.** + **Perform transient and stable transfections for protein expression in mammalian cells (CHO, HEK293).** + Ability to multi-task different protocols at once, handle concurrent projects simultaneously with high attention to detail. **Nice to haves/preferred:** + Experience with high-throughput systems and automation. + Experience with batch purification using a variety of scaffolds and basic QC. + Industry experience is preferred. **Software skills:** + **Basic knowledge on biological data collection and analysis tools (Vicell, metabolite analyzers) and computer applications such as ELN, MS Word, MS Excel and MS PowerPoint.** + **Experience with data and reagent management software such as Dotmatics, Benchling, Spotfire, etc is preferred.** **Skills/techniques needed:** + **Excellent aseptic techniques and familiarity with principles of sterilization/disinfection.** + Strong communication, organizational, and interpersonal skills. + Able to maintain a “can-do” attitude and act with a sense of urgency to complete innovative projects while being flexible in work priorities based on business needs. + Understand and adhere to corporate standards regarding code of conduct, safety, appropriate handling of materials + **Able to troubleshoot cell culture system and think critically about experiments.** + Must be comfortable with physical activities of constant standing, walking, and sitting with arm stretching into biosafety cabinets for cell culture work. **Summary:** + Our client is Discovery Biologics, and they are on a quest for cures and are committed to be the world's premier, most research-intensive biopharmaceutical company. They identify and target steps in disease mechanisms or pathways that could be inhibited or enhanced. Their goal is to isolate a compound that is effective against a disease target. Using innovative thinking, state-of-the-art facilities and robust scientific methodology we collaborate to discover the next medical breakthrough. + Their teams combine leading drug discovery capabilities and world-class R&D with the purpose of turning breakthrough science into life-changing medicines. + They are looking for a motivated scientist to be an integral part of their Protein Science department within Discovery Biologics. + The successful candidate will be involved in multiple aspects of the production of recombinant proteins, monoclonal antibodies, multispecific scaffolds, and novel protein modalities to enable all aspects of Early Discovery. + Ideally, the candidate will be able to execute and develop on techniques involving recombinant protein expression in CHO cells using both transient and stable-pool techniques. + Additionally, the candidate should have foundational experience in purification and QC, along with a proven ability to accurately record and present data. **Job responsibilities in detail:** + Maintain and propagate CHO cells in suspension culture independently to support ongoing projects in a cell culture lab. + Perform transient and stable transfections for protein expression in mammalian cells (CHO, HEK293). Express and batch purify a variety of proteins (monoclonal, bispecific antibodies, VHHs, Fabs, Fc-Fusions, etc.) using both resin and magnetic-bead immunoprecipitation methods. + Execute fed-batch experiments in a variety of platforms: shake flasks 125ml to 5L, tube spins and multi-well plates. + Perform analytical measurements for cell growth, metabolite measurements and HPLC analysis of titers from supernatant. + Analyze purified proteins using techniques: SDS-PAGE, HPLC, UPLC, CESDS, Nano-drop, UV-Vis. + Maintain and troubleshoot mammalian cell culture systems. + Document protein production processes, prepare reports and communicate results + ELN documentation. If this is a role that interests you and you’d like to learn more, click apply now and a recruiter will be in touch with you to discuss this great opportunity. We look forward to speaking with you! **About ManpowerGroup, Parent Company of:** **Manpower, Experis, Talent Solutions, and Jefferson Wells** _ManpowerGroup® (NYSE: MAN), the leading global workforce solutions company, helps organizations transform in a fast-changing world of work by sourcing, assessing, developing, and managing the talent that enables them to win. We develop innovative solutions for hundreds of thousands of organizations every year, providing them with skilled talent while finding meaningful, sustainable employment for millions of people across a wide range of industries and skills. Our expert family of brands –_ **_Manpower, Experis, Talent Solutions, and Jefferson Wells_** _–_ creates substantial value for candidates and clients across more than 75 countries and territories and has done so for over 70 years. 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