**Job Description** **Purpose of the research project(s):** The RSE-assistant will provide technical support to RSEs exploring mechanisms underlying prion appearance. **Major Duties and Responsibilities:** 1. Media, reagent, and glassware preparation for laboratory research; cleaning and sterilization of glassware for culture growth and liquid transfers; preparation of solid medium for yeast and bacterial growth, preparation of liquid growth media for yeast and bacterial growth; preparation of buffers for gel electrophoresis and western blotting; preparation of reagents and reagent aliquots for cell lysis, genomic preps, cloning, etc. 30% 2. Ordering laboratory supplies and maintaining inventory and equipment; liaising with department administration and external vendors for pricing and purchasing. 10% 3. Provide experimental support to Research Scientist/Engineers; work closely with senior RSEs to perform experiments investigating prion dynamics according to established protocols; troubleshoot, amend, and optimize experimental protocols with guidance from senior RSEs; read the literature regularly to gain understanding of prion dynamics in yeast and mammalian systems and so as to develop/design appropriate methodology for experiments; make and maintain records of all experiments performed and data collected; analyze results, develop data-informed conclusions and hypotheses. 50% 4. Attend weekly biochemistry departmental seminars and other departmental (e.g. genome science, lab medicine, etc.) seminars related to our field of interest; attend and participate/present at the weekly joint group meeting with the Klevitt lab; participate in departmental student/postdoc research presentations/talks; summarize data into coherent story for weekly presentation with colleagues and lab manager. 10% **Minimum Requirements:** Bachelor's Degree in Biochemistry, Biology and Chemistry + less than one year of experience in related field. Equivalent education and/or experience may substitute for minimum qualifications except when there are legal requirements, such as a license, certification, and/or registration. **Additional Requirements:** 1. Work and think independently. 2. Follow written protocols meticulously and maintain a detailed laboratory notebook. 3. Willingness to work flexible hours. 4. Strong math and language skills. 5. Basic computer knowledge required (word processing, spreadsheets, database searches). 6. Knowledge of molecular biology, genetic and/or biochemistry concepts. **Desired Qualifications:** Experience in laboratory techniques including: 1. Preparation of reagents for personal use, preparation of stock reagents and media for the laboratory, place orders for laboratory reagents and supplies with external vendors. 2. Recombinant DNA research (cloning, mutagenesis). 3. Genetic crosses, construction of recombinant yeast strains, genetic screening in yeast. 4. Protein biochemistry (protein expression, extraction, purification, gel electrophoresis, immunoblotting, immunoprecipitation). **Compensation, Benefits and Position Details** **Pay Range Minimum:** $52,692.00 annual **Pay Range Maximum:** $81,012.00 annual **Other Compensation:** - **Benefits:** For information about benefits for this position, visit https://www.washington.edu/jobs/benefits-for-uw-staff/ **Shift:** First Shift (United States of America) **Temporary or Regular?** This is a regular position **FTE (Full-Time Equivalent):** 100.00% **Union/Bargaining Unit:** UAW Research **About the UW** Working at the University of Washington provides a unique opportunity to change lives – on our campuses, in our state and around the world. UW employees bring their boundless energy, creative problem-solving skills and dedication to building stronger minds and a healthier world. In return, they enjoy outstanding benefits, opportunities for professional growth and the chance to work in an environment known for its diversity, intellectual excitement, artistic pursuits and natural beauty. **Our Commitment** The University of Washington is committed to fostering an inclusive, respectful and welcoming community for all. As an equal opportunity employer, the University considers applicants for employment without regard to race, color, creed, religion, national origin, citizenship, sex, pregnancy, age, marital status, sexual orientation, gender identity or expression, genetic information, disability, or veteran status consistent with UW Executive Order No. 81 (https://policy.uw.edu/directory/po/executive-orders/eo-81-prohibiting-discrimination-harassment-and-sexual-misconduct/) . To request disability accommodation in the application process, contact the Disability Services Office at 206-543-6450 or dso@uw.edu . Applicants considered for this position will be required to disclose if they are the subject of any substantiated findings or current investigations related to sexual misconduct at their current employment and past employment. Disclosure is required under Washington state law (https://app.leg.wa.gov/RCW/default.aspx?cite=28B.112.080) . University of Washington is an affirmative action and equal opportunity employer. All qualified applicants will receive consideration for employment without regard to, among other things, race, religion, color, national origin, sexual orientation, gender identity, sex, age, protected veteran or disabled status, or genetic information.