Req #: 245624 Department: NEUROLOGY Appointing Department Web Address: https://neurology.uw.edu/ Posting Date: 05/12/2025 Closing Info: Open Until Filled Salary: $5,160 - $9,465 per month Shift: First Shift Notes: As a UW employee, you will enjoy generous benefits and work/life programs. For a complete description of our benefits for this position, please visit our website, click here. (https://hr.uw.edu/benefits/wp-content/uploads/sites/3/2018/02/benefits-professional-staff-librarians-academic-contract-covered-exempt-20250130-a11y.pdf) As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem-solving skills, and dedication to build stronger minds and a healthier world. UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits, and natural beauty. **The Department of Neurology has an outstanding opportunity for a Research Scientist/Engineer 2 to join their team.** **POSITION PURPOSE** Molecular genetics and gene therapy and find a treatment for muscular dystrophy. **DUTIES AND RESPONSIBILITIES** Mouse Husbandry – 25% + Maintaining a mouse colony (including genotyping, record keeping, interfacing with vivarium staff), planning and executing mouse crosses to generate subject mice for experiments. + Treatment of mice with experimental reagents (e.g. via intraperitoneal, intravenous or subcutaneous injection). + Sacrifice mice and isolation/preparation of tissue samples.Analysis of mice – 25% + Conduct experimental studies on treated and control mice. + Physiology of muscles (measuring mechanical properties such as force development). + DNA and protein quantitation using antibodies, PCR and immunostaining.Molecular Biology – 40% + Maintaining immortalized cells in culture (e.g. HEK293, HeLa, C2, MM14, etc). + Isolation of biomolecules (DNA, RNA and Protein) from tissue and cell samples. + Quantification of target gene expression with quantitative real time PCR (QRT-PCR). + Quantification of target protein expression with western blots. + Staining of tissue slides for immunohistochemistry. + Analysis of immunohistochemical images using existing protocols in the ImageJ software package. + Conducting 96-well micro-plate based assays (e.g. QPCR, Bradford, ELISA, etc.). + Propagation of plasmids, generation of stocks via miniprep or related techniques. + Generation of sequencing libraries (snRNAseq, CUT&RUN, ATACseq, etc). + Cloning and sequencing of plasmids for vector generation (PCR, restriction digests, DNA sequencing, gel electrophoresis).Supervision and training – 10% + Supervise undergraduate student helpers. + Maintain lab training records. **MINIMUM REQUIREMENTS** + Bachelor’s degree in engineering or scientific discipline or a related field and two years of relevant experience. **Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration.** **DESIRED QUALIFICATIONS** + Molecular biology skills (DNA/RNA, cloning, PCR, DNA sequencing). + Experience with antibodies and tissue histology techniques. + Able to read and explain scientific publications. + Experience with small lab animals (mice). + Experience with viral vectors, especially AAV, use and analysis. **Application Process:** The application process may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment, you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are access ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed. University of Washington is an affirmative action and equal opportunity employer. All qualified applicants will receive consideration for employment without regard to, among other things, race, religion, color, national origin, sexual orientation, gender identity, sex, age, protected veteran or disabled status, or genetic information.